Molzym GmbH & Co. KG

Workshops

We are offering the following workshops:

Workshop I	Workshop II
“Automation in Culture-Independent, Sequencing-Based Pathogen Diagnosis”	“Unbiased, Direct Identification of Microorganisms in Clinical Samples”

Workshop I:

“Automation in Culture-Independent, Sequencing-Based Pathogen Diagnosis”

Automation is an important issue in daily routine diagnosis. Molzym provides an unique automated DNA extraction specifically adapted to the unbiased, culture-independent diagnosis of bacterial and fungal pathogens. The one-day course will confer theoritical and practical skills in the automated sample preparation and the Real-Time PCR analysis of eluates for microbial DNA based on Micro-Dx™ CE IVD:

Basics of automated DNA extraction and unbiased, pathogen diagnosis
Introduction to the SelectNA™plus robot
Pre-treatment of tissue samples
Loading of the robot with samples
Real-Time PCR analysis of bacterial DNA
Interpretation of Real-Time PCR melting curves
Analyses and interpretation of sequencing results

Program:

09:00 Start:

Welcome
Introduction to Molzym’s diagnostic system, Micro-Dx™

9:30 - 12:30 Practical part I:

Introduction to the SelectNA™plus robot
Loading of the robot with samples and automated DNA isolation
16S rRNA Real-Time-PCR

12:30 - 13:30 Lunch

13:30 - 16:00 Practical part II:

Interpretation of melting curves
Analysis and interpretation of sequencing results by different online BLAST tools
Overview: Clinical samples and case studies

16:00 - 16:30 Open discussion

16.30 End of the workshop

Registration:

Tel.: +49 (0) 421 / 69 61 62 17
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Attendance fee: 150,- € including lunch and coffee breaks

Max. attendees: 4

Workshop II:

“Unbiased, Direct Identification of Microorganisms in Clinical Samples”

In this one-day professional training you get a detailed theoretical and practical insight into the procedure of detection and identification of pathogens by Molzym’s diagnostic test, SepsiTest™-UMD CE IVD. The course includes:

Basics of unbiased, broad-range pathogen diagnosis
Sample pre-treatment for removal of human DNA
Preparation of microbial DNA
Real-Time PCR analysis of bacterial DNA
Interpretation of Real-Time PCR melting curves
Analyses and interpretation of sequencing results